This week, in our biweekly meeting for The Bridge, Richelle and I talked about how neuroscientists slice brains for physiology experiments in labs. Richelle had a lot of great questions about how we slice brains, what the equipment looks like, how we keep the neurons alive, and how long the process takes. After chatting with Richelle, I decided to take some photos of the dissecting and slicing equipment and put together some captions. My hope is that these images will be useful for Richelle when she creates images to go with my text for our collaborative project. Here are some of the photos and captions that I sent Richelle:
This is the chamber that the brain slices recover in for about 1.5 hours after getting sliced. If you look carefully in the bottom right corner of the photo, you can see three slices lined up on top of the silver netting. The slices incubate here inside this box with a solution that has nutrients to keep them alive. In order to stay alive, the neurons on these slices also require oxygen, so we have gas tanks that bubble oxygen into the chamber.
Check back next week for another update. Cheers!